Soybean variety S03-95341-A98-60618

ABSTRACT

The present invention is in the field of soybean variety S03-95341-A98-60618 breeding and development. The present invention particularly relates to the soybean variety S03-95341-A98-60618 and its progeny, and methods of making S03-95341-A98-60618.

THE FIELD OF THE INVENTION

The present invention is in the field of soybean varietyS03-95341-A98-60618 breeding and development. The present inventionparticularly relates to the soybean variety S03-95341-A98-60618 and itsprogeny, and methods of making.

BACKGROUND OF THE INVENTION

The breeding and development of crops has been ongoing across the last1000 years. The pace of this development in soybeans, as an animalfoodstuff and as an oil seed has dramatically increased in the last onehundred years. Planned programs of plant breeding have increased thegrowth, yield and environmental hardiness of the soybean germplasm. Dueto the sexual reproduction traits of the soybean the plant is basicallyself-pollinating. A self-pollinating plant permits pollen from oneflower to be transferred to the same or another flower of the sameplant. Cross-pollination occurs when the flower is pollinated withpollen from a different plant. This is a rare occurrence in nature.

Thus the growth and development of new soybean germplasm requiresintervention by the breeder in the pollination of the soybean. Thebreeders' selections of methods of intervening in the pollination dependon the type of trait that is being selected. Soybeans are developed fora number of different types of traits morphological (form andstructure), phenotypical, for growth habit, daylength temperaturerequirements to initiate floral or reproductive development yield. Thegenetic complexity of the trait drives the breeding method. Backcrossbreeding is employed when the cultivar that is being bred has a fairlyfull profile of desirable traits, but lack one or two traits that arehighly inheritable. Backcrossing is often employed to move diseaseresistance, insect resistance and transgenes (hereinafter DNA which areintroduced into the original ancestor germplasm by a transformationmethod) into other varieties.

When the variety is being employed to develop a new variety or animproved variety the selection methods include pedigrees, recurrent,modified and mass selection and backcrossing. Each of these selectiontechniques is employed with the efficiency of the breeding procedure asthe driver. The breeding procedure requires a continuous evaluation ofthe success of the breeding program. The success is measured by yieldincrease, commercial appeal and environmental adaptability of thedeveloped germplasm.

New varieties must be tested thoroughly to compare the development withthe commercially available soybeans. This testing usually requires atleast two years and up to six years of comparisons with other commercialsoybeans. Varieties that lack the entire desirable package of traits canbe used as parents in new populations for further selection. Thebreeding and associated testing process is 8 to 12 years' progressiontoward a new variety. Thousands of lines are produced and limited linesare selected in each step of the process. Thus the breeding system islike a funnel with numerous lines and selections in the first few yearsand fewer and fewer lines in the middle years until one line is selectedfor the final development testing.

The selected line or variety will be evaluated for it's the growth anddevelopment and yield. These traits of a soybean are a result of thevarieties genetic potential interacting with its environment. Allvarieties have a maximum yield potential that is predetermined by itsgenetics. This hypothetical potential for yield is only obtained whenthe environmental conditions are perfect. Since prefect growthconditions do not exist field experimentation is necessary to providethe environmental influence and to measure its effect on the developmentand yield of the soybean. The breeder attempts to select for goodsoybean yield potential under a number of different environmentalconditions.

Selecting for good soybean yield potential under a number of differentenvironmental conditions is a process that requires planning, analysisof data in a number of seasons. Identification of the varieties thatcarry a superior combination of traits that provides this consistentyield potential is a complex science. Other plant traits, unusualweather patterns, diseases, and insect damage often mask the genotypictraits. One widely employed method of identifying a superior plant is toobserve its performance relative to commercial and experimental plantsin replicated studies. These types of studies give more certainty to thegenetic potential and value of the plant.

The goal of the soybean plant breeder is to produce unique and newsoybeans and progeny of the soybeans. To accomplish this the plantbreeder painstakingly crosses two or more varieties or germplasm. Thenthe results of this cross is repeatedly selfed or backcrossed to producenew genetic patterns. Additionally, the breeder can introduce mutationsinto the genetic material. These can alter herbicide resistance, fattyacid compositions, and amino acid compositions of the seeds and thelike. Fortunately, through transformation in combination with breedingthe plant breeder can alter or introduce some genetic alleles into thebreeding material.

These capabilities are widening the potential uses and markets for thevarious products and by-products of oil seed plants such as soybean. Oneof the products of soybeans is the oil of the seed. Soybean oil isemployed in a number of retail products. Soybean meal is also used infood and animal feedstuffs. The traits a breeder selects for can bedriven by the ultimate goals of the end user of the product. Thus if thegoals of the end user is to produce an oil with a high level of oleicacid and a lower level of linoleic acid then the breeder may drive thegenetics toward levels of fatty acids and accept some lesser yieldpotentials or other less desirable agronomic traits.

Regardless of the market characteristics of the plant most breedingproceeds along a similar path on a yearly basis. The breeder annuallyselects the germplasm to advance on into further development. Thisgermplasm is grown in different locations at different altitudes, indifferent climates and subjected to different soil conditions. Based onthe datum collected from these tests individual plants are selectedduring the end of the growing season. Due to the number of genes withineach chromosome millions of genetic combinations exist in the breeders'experimental soybean material. This genetic diversity is so vast that abreeder cannot produce the same two cultivars twice using the exact samematerial. Thus the developing a single variety of useful commercialsoybean germplasm is highly unpredictable, and requires intensiveresearch.

The development of new soybeans comes through breeding techniques suchas recurrent selection, mass selections, backcrossing, single seeddescent and multiple seed procedure that is used to save labor costs.Other breeding methods are taught in several soybean textbooks.

The development of soybean cultivars most often requires the developmentof hybrid crosses (some exceptions being initial development of mutantsdirectly through the use of the mutating agent or transformants madedirectly through transformation methods) and the selection of progenytherefrom. Hybrids can be achieved by manual manipulation of the sexualorgans of the soybean or by the use of male sterility systems. Thebreeder attempts to identify true hybrids by a readily identifiabletrait. These hybrids are then selected and repeatedly selfed andselected to form new homozygous lines from the heterozygous hybrids.

Outcrossing to a number of different parents creates breedingpopulations of fairly heterozygous populations. These populations areproduced and used in pedigree breeding and recurrent selection. Pedigreebreeding is commonly used with two parents which possess favorable,complementary traits. The parents are crossed to form a F1 hybrid. Theprogeny of the F1 hybrid is selected from this the best individuals F2are selected; this selection process is repeated in the F3 and F4generations. The inbreeding is carried forward and at F5-F7 the bestlines are selected and tested in the development stage for potentialusefulness.

Mass and recurrent selection can be used to improve populations. Severalparents are intercrossed and plants are selected based on selectedcharacteristics like superiority or excellent progeny.

In backcross breeding a genetic allele or loci is transferred into adesirable homozygous recurrent parent. The trait is in the donor parentand is tracked into the recurrent parent. The resultant plant is likethe recurrent parent with the new desired allele or loci.

The single-seed descent method involves use of a segregating plantpopulation for harvest of one seed per plant. Each seed sample isplanted and the next generation is formed. When the F2 lines areadvanced to F6 each plant will be derived from a different F2. Thepopulation will decline due to failure of some seeds, so not all F2plants will be represented in the progeny.

Soybean Glycine max (L) is an important oil seed crop and a valuablefield crop.

SUMMARY OF THE INVENTION

One embodiment of the invention relates to seed of a soybean cultivardesignated S03-95341-A9860618. The invention relates to the plant fromthe seed designated S03-95341-A98-60618, or the plant parts includingovule, a tissue culture of regenerable cells, cells or protoplasts beingfrom a tissue selected from the group consisting of leaves, pollen,embryos, meristematic cells, roots, root tips, anthers, flowers, seeds,stems and pods and pollen thereof, produced by growing the seed.

The invention in one aspect covers a soybean plant, or parts thereof,having all of the physiological and morphological characteristics of thesoybean plant.

Another aspect of this invention is the soybean plant seed or derivedprogeny which contains a transgene (which include DNA whether or not itis a full or partial sequence) affecting herbicide resistance, insectresistance, resistance to disease, nematodes, male sterility, andaltered oil or amino acids or other nutritional quality.

The present invention further covers a method for producing a soybeanseed with the steps of crossing at least two parent soybean plants andharvesting the hybrid soybean seed, wherein at least one parent soybeanplant is the present invention. In another aspect of the inventioncovers the hybrid soybean seed and the progeny soybean plant andresultant seed, or parts thereof from the hybrid seed or plant or itsprogeny.

In an additional aspect the invention covers a method for producing asoybean progeny from the invention by crossing soybean lineS03-95341-A98-60618 with a second soybean plant to yield progeny soybeanseed and then growing progeny soybean seed to develop a derived soybeanline.

Yet another aspect of the invention covers a method for a breedingprogram using plant breeding techniques which employ the soybean plantS03-95341-A98-60618 as plant breeding material and performing breedingby selection techniques, backcrossing, pedigree breeding, markerenhanced selection, mutation and transformation.

Soybean Data Collection Core Traits Abbr. Description Timing Scale HilaHC Phenotypic color; G = Gray; All experiments. BR = Brown; IB =Imperfect Black; Bl = Black; Y = Yellow; BF = Buff; IY = ImperfectYellow; X = Mix Flower FC Phenotypic color; W = White; All experiments.P = Purple; X = Mix Pod POD Phenotypic color; T = Tan; All experiments.B = Brown; X = Mixed Pubescence PUB Phenotypic color; G = Gray; Allexperiments. T = Tawny; LT = Light Tawny; X = Mixed GWT GWT Grainweight/plot Harvest Pounds H2O H20 Grain moisture/plot Harvest %moisture Necessary Traits Abbr. Timing Scale Hypocotyl Elongation HYPOReplicated Nursery May to June 1 to 5 (1 = best) Seedling EstablishmentEMG 4 locations/test VE-V1 1 to 5 (1 = best) Maturity MAT 4locations/test Taken in days after Aug. 31 Plant Height PLTHT 4locations/test Harvest Taken in inches Branching BR 4 locations/testR8-Harvest 1 to 5 (1 = no branch) Agronomic Traits Abbr. OpportunisticTiming Scale Green Lodging GLOD Where differential occurs. R5 to R6 1 =All erect 2 = 67° 3 = 45° 4 = 22° 5 = 0°

Stem Lodging LOD Where differential occurs Harvest 1 = All erect 2 = 67°3 = 45° 4 = 22° 5 = 0°

Shatter SHAT Where differential occurs Harvest 1 to 5 (1 = best)Opportunistic Disease Ratings Abbr. Key Maturities Scale PhytophthoraRoot Rot PFT All 1 to 5 (1 = best) Brown Stem Rot BSR G0 to EGIII 1 to 5(1 = best) Sclerotinia White Mold SWM G0 to EGIII 1 to 5 (1 = best)Sudden Death Syndrome SDS EGIII to GVII 1 to 5 (1 = best) Stem CankerSTMC MGII to GVII 1 to 5 (1 = best) Charcoal Rot CROT LGIII to GVII 1 to5 (1 = best) Frog Eye FROG EGIII to GVII 1 to 5 (1 = best) DiseaseNurseries Abbr. Path Timing Scale Iron Deficiency Chlorosis IDC InternalField Nursery June-July 1 to 5 (1 = best) Soybean Cyst Nematode SCN Race3 Internal Nursery Nov-April # = the race 30d cycle R3 is resistant torace 3 MR14 is moderately resistant to race 14 R-MR-MS-S R = resistant S= susceptible M = moderately Phytophthora Root Rot PRR PFT or PGR 1 to 5(1 = best) for field tolerance and resistance gene Rps 1a, Rps 1b, Rps1c, Rps 1k, etc for specific genes. Sudden Death Syndrome SDS DiseaseSeverity Index or DSI. Brown Stem Rot BSR 1 to 5 (1 = best) Root KnotNematode RKN R-MR-MS-S R = resistant S = susceptible M = moderately StemCanker STMC 1 to 5 (1 = best) Herbicide Evalutation Abbr. SulfentraxoneSULF Sensitive, Tolerant Metributzin MET Sensitive, Tolerant ***All datato default to a “.” (period) when data is not observed

DETAILED DESCRIPTION Trait Definitions

Hypocotyl Elongation (HYPO) A rating of a variety's hypocotyl extensionafter germination when planted at a 5″ depth in sand and maintained awarm germination environment for 10 days.

Seedling Establishment (EMG) A rating of the uniform establishment andgrowth of seedlings.

Maturity (MAT) The number of days after Aug. 31 when 95% of the mainstem pods in the plot have reached their mature color.

Peroxidase Activity (Perox)—seed protein peroxidase activity is definedas a chemical taxonomic technique to separate cultivars based on thepresence or absence of the peroxidase enzyme in the seed coat. Ratingsare POS=positive for peroxidase enzyme or NEG=negative for peroxidaseenzyme.

Plant Height (PLTHT) The average measured plant height in inches.

Branching (BRANCH) Rating of the number of branches and their relativeimportance to yield. Taken at growth expressive locations.

Green Lodging (GLODGE) Rating based on the average of plants leaningfrom vertical in R5 to R6 stage.

Stem Lodging (LODGE) Rating based on the average of plants leaning fromvertical at harvest.

Shatter (SHAT) Rating of pre-harvest loses based on amount of plantswith open pods.

Iron Deficiency Chlorosis (IDC) A composite rating of Yellow Flash,Green-up, and Stunting in HpH soil.

Phytophthora Root Rot (PGR) or (PFT) Greenhouse pot—root dip method forPFT and hypodermic needle method for rating PGR.

Root Knot Nematode (RKN) Greenhouse screen—30 day screen using infestedsoil. Rating Scale based upon female reproduction index on a susceptiblecheck set where <10%=R; <30%=MR; <60%=MS; >60%=S.

Stem Canker (STC) Based on number of lesions, scale 1-5.

Sulfentrazone (SULF) Authority™ (commercial herbicide) Greenhousenursery rating damage of multiple rates.

Metributzin (MET) Greenhouse nursery rating damage of multiple rates.

Definitions of Staging of Development

The plant development staging system employed divides stages asvegetative (V) and reproductive (R). This system accurately identifiesthe stages of a soybean plant. However, all plants in a given field willnot be in the stage at the same time. Each specific V or R stage isdefined as when 50% or more of the plants in the field are in or beyondthat stage.

The first two stages of V are designated a VE (emergence) and VC(cotyledon stage). Subdivisions of the V stages are then designatednumerically as V1, V2, V3 through V (n) The last V stage is designatedas V (n), where (n) represents the number for the last node stage of thespecific variety. The (n) will vary with variety and environment. Theeight subdivisions of the reproductive stages (R) states are alsodesignated numerically. R1=beginning bloom; R2=full bloom; R3=beginningpod; R4=full pod; R5=beginning seed; R6=Full Seed; R7=beginningmaturity; R8=Full maturity.

BROWN STEM ROT (BSR)-This disease is caused by the fungus Phialophoragregata. The disease is a late-season, cool-temperature, soilborne whichin appropriate favorable weather can cause up to 30 percent yield lossesin soybean fields. For purposes of these tests the information isgathered in a greenhouse with a plant in a pot then a root dip procedureis employed.

SUDDEN DEATH SYNDROME (SDS)—This disease is caused by slow-rowingstrains of Fursarium solani that produce bluish pigments in culture. Thedisease is mid- to late season soil borne and occurs in soybean fieldswith high yield potential. Yield losses may be total/severely affectedfields. Sudden Death Syndrome (SDS) is based on leaf area affected,scale used for these tests is 1-5.

SOYBEAN CYST NEMATODE—The Soybean Cyst Nematode (SCN) Heteroderaglycines, is a small plant-parasitic roundworm that attacks the roots ofsoybeans.

Soybean Cyst Nematode (SCN) for purposes of these tests are done as agreenhouse screen—30 day screen using infested soil. Rating Scale basedupon female reproduction index on a susceptible check set where <10%=R;<30%=MR; <60%=MS; >60%=S. In priority order, the screening racesinclude: 3, 14, & 1.

MATURITY DATE. Plants are considered mature when 95% of the pods havereached their mature color. The number of days is either calculated fromSeptember 1 or from the planting date. (MR#) wherein # equals days.

RELATIVE MATURITY GROUP (RM). Industry Standard for varieties groups,based day length or latitude. Long day length (northern areas in theNorthern Hemisphere) are classified as (Groups 000,00,0,) and extend tovery short day lengths variety groups (southern areas in NorthernHemisphere) classified as (Groups VII,VIII, IX).

SEED YIELD (Bushels/Acre). The yield in bushels/acre is the actual yieldof the grain at harvest.

SPECIFICATION AMENDMENT

SHATTERING. The rate of pod dehiscence prior to harvest. Pod dehiscenceinvolves beans dropping out of the pods. Shatter (SHAT) for these teststhe rating of pre-harvest loses is based on amount of plants with openpods.

PLANT. Means the plant, the plant's cells, plant protoplasts, plantcells of tissue culture from which soybean plants can be regenerated,plant calli, plant clumps, and plant cells that are intact in plants orparts of the plants, such as pollen, nodes, roots, flowers, seeds, pods,leaves, stems, and the like.

The present invention is 95341-A98-60618. This soybean is developed forits use of the beans. 95341-A98-60618 is a 2.9 relative maturity.95341-A98-60618 is adapted to both wide and narrow row spacing in thelate group 2 maturities. This variety is an excellent choice for highperformance and provides excellent brown stem rot resistance. This lineis growth and is adaptable to moisture stress. This cultivar's generalarea of adaptation in the U.S. includes most soil types found inNorthern to Central Nebraska, Northern Illinios, Central Iowa, NorthernIndiana.

The traits of the invention are listed below.

Trait RM 2.9 HR-herbicide resistance — Flower Color P Pubescene Color TPod Color [T] B Hila Color BL Lust Dull Perox NEG PFT 4.0 Hypo 2.00 IDC3.7 BSR 1.7 SDS 2.0

The instant invention provides methods and composition relating toplants, seeds and derivatives of the soybean cultivarS03-95341-A98-60618. Soybean cultivar S03-95341-A98-60618 has superiorcharacteristics. The S03-95341-A98-60618 line has been selfed sufficientnumber of generations to provide a stable and uniform plant variety.

Cultivar S03-95341-A98-60618 shows no variants other than expected dueto environment or that normally would occur for almost anycharacteristic during the course of repeated sexual reproduction. Someof the criteria used to select in various generations include: seedyield, lodging resistance, emergence, appearance, disease tolerance,maturity, plant height, maturity and shattering data.

The inventor believes that S03-95341-A98-60618 is similar to thecomparison varieties. However, as shown in the tables,S03-95341-A98-60618 differs from these cultivars.

Direct comparisons were made between S03-95341-A98-60618 and thesecompeting commercial varieties. Traits measured included yield,maturity, moisture, lodging, plant height, field emergence, protein andoil. The results of the comparison are presented in below. The number oftests in which the varieties were compared is shown. The deviation ordifference of the results, T-value and the traits which showed asignificant difference and the level of that significance are in thefirst table. The second table indicates the advantage (positive number)or disadvantage (negative number) of the present invention when comparedto the commercial lines.

The present invention S03-95341-A98-60618 can carry genetic engineeredrecombinant genetic material to give improved traits or qualities to thesoybean. For example, but not limitation, the present invention cancarry, the glyphosate resistance gene for herbicide resistance as taughtin the Dekalb U.S. patent or STS mutation for herbicide resistance.Additional traits carried in transgenes or mutation can be transferredinto the present invention. Some of these genes include genes that givedisease resistance to sclerotina such as the a oxalate oxidase (Ox Ox)gene as taught in PCT/FR92/00195 Rhone Polunc and/or an Ox Decarboxylategene for disease resistance or genes designed to alter the soybean oilwithin the seed such as desaturase genes or genes designed to alter thesoybean's amino acid characteristics. This line can be crossed withanother soybean line which carries a gene that acts to provide herbicideresistance or alter the saturated and/or unsaturated fatty acid contentof the oil within the seed, or the amino acid profile of the seed.

The present invention S03-95341-A98-60618 is employed in a number ofplot repetitions to establish trait characteristics.

External Name RM Flower Hilum BSR SDS IDC S03- 2.9 P BL 1.7 2.0 3.795341- A98- 60618 A2553 2.5 — IB 2.0 3.5 P93B15 3.1 P BR 4.9 S30-Y8 3 PIB 1.0 4.0

-   HR=herbicide resistance-   RR=glyphosate resistance from Monsanto

The traits of the present invention differ from the comparisoncommercial soybean lines in a combination of a number of traits. Thepresent invention shows a different hilum than each of the other lines.The present invention has nice brown stem root resistance and carriessome levels of tolerance to Sudden Death Syndrome. Additionally thepresent invention shows better IDC ratings than does any of the othercommercial lines except A2553.

The present invention S03-95341-A98-60618 is employed in a trialling fora number of characteristics. These tests allow the usefulness of theinvention to be shown in light of the environmental geneticinteractions.

Appear- Ent Yld Moist ance Branch S03-95341-A98-60618 vs A2553S03-95341-A98-60618 65.3 11.7 2 2.8 A2553 61.7 11.7 2.4 3.1 # LOCS 15 155 2 Diff 3.6 0 −0.4 −0.4 Std 7.6 0.3 1.1 0.5 T-val 1.84 0.02 −0.78 −1Prob 0.086* 0.986 0.477 0.5 S03-95341-A98-60618 vs P93B15S03-95341-A98-60618 65.3 11.7 2 2.8 P93B15 66.1 11.6 2 3.6 # LOCS 15 155 2 Diff −0.8 0.1 0 −0.9 Std 8.1 0.1 0 0.2 T-val −0.38 1.99 −7 Prob0.712 0.067* 0.090* S03-95341-A98-60618 vs S30-Y8 S03-95341-A98-6061865.3 11.7 2 2.8 S30-Y8 63.9 11.8 2.1 3.6 # LOCS 15 15 5 2 Diff 1.4 −0.1−0.1 −0.9 Std 7.3 0.3 0.9 0.2 T-val 0.74 −1.89 −0.25 −7 Prob 0.41 0.080*0.815 0.090* *Significant at the 0.10 level **Significant at the 0.05level ***Significant at the 0.01 level Moist = Moisture Yld = Yield

Emer- Ent BSR gence Lg Mat S03-95341-A98-60618 vs A2553S03-95341-A98-60618 2 1.5 20.5 A2553 2.3 1.7 17.1 # LOCS 0 2 9 7 Diff−0.3 −0.2 3.3 Std 0.4 0.4 3.3 T-val −1 −1.15 2.69 Prob 0.5 0.282 0.036**S03-95341-A98-60618 vs P93B15 S03-95341-A98-60618 2 1.5 20.5 P93B15 2.51.7 22.9 # LOCS 0 2 9 7 Diff −0.5 −0.2 −2.4 Std 0 0.4 2.3 T-val −1.15−2.78 Prob 0.282 0.032** S03-95341-A98-60618 vs S30-Y8S03-95341-A98-60618 2 1.5 20.5 S30-Y8 1.7 1.3 21.5 # LOCS 0 2 9 7 Diff0.3 0.2 −1 Std 0.3 0.4 2.9 T-val 1.26 1.44 −0.93 Prob 0.427 0.189 0.388Lg = lodging Mat = maturity in days after September 1

Ent Pltht PRR Shatter IDC S03-95341-A98-60618 vs A2553S03-95341-A98-60618 35.7 4.4 A2553 32.6 4 # LOCS 5 0 0 4 Diff 3.1 0.5Std 3 0.5 T-val 2.27 1.94 Prob 0.085* 0.147 S03-95341-A98-60618 vsP93B15 S03-95341-A98-60618 35.7 4.4 P93B15 33.6 4.6 # LOCS 5 0 0 4 Diff2.1 −0.2 Std 2.2 0.2 T-val 2.09 −1.57 Prob 0.105 0.215S03-95341-A98-60618 vs S30-Y8 S03-95341-A98-60618 35.7 4.4 S30-Y8 34.44.6 # LOCS 5 0 0 4 Diff 1.3 −0.1 Std 2.9 0.3 T-val 1.03 −0.93 Prob 0.360.423 Pltht = plant height

The present invention is providing yield and moisture consistent withthe comparison commercial lines. Each of these lines have their ownpositive traits. Each of these lines are different from the presentinvention. The lodging levels of the present invention in comparison tothe other lines very similar without any significant difference. The IDCrating is slightly lower than two of the other lines though notsignificantly lower than the commercial lines. The yield and other datais a snapshot of each of these lines' results in the specificenvironment and will differ when other environmental interactions aremeasured. The present invention is being compared with lines of similarmaturities which are quite like the maturity of the present invention.The maturity is measured in the number of days after September 1 untilmaturity is reached.

This S03-95341-A98-60618 invention was compared with the comparisonsoybean product for certain grain quality traits. The data in the firstcolumn shows that seed per pound of the present invention are similar toA2553 but different from S30-Y8. The protein levels found in the presentinvention are consistently higher in these results. The oil level in theseed of the present invention is on the lower side comparatively.

sds per lb Protein oil S03-95341-A98- 3100-3900 34.00 18.60 60618 A25533000-4100 33.20 19.00 S30-Y8 3100-3600 33.00 18.70

This invention also is directed to methods for producing a new soybeanplant by crossing a first parent plant with a second parent plantwherein the first or second parent plant is the present invention.Additionally, the present invention maybe used in the varietydevelopment process to derive progeny in a breeding population orcrossing. Further, both first and second parent plants can come from thesoybean line S03-95341-A98-60618. A variety of breeding methods can beselected depending on the mode of reproduction, the trait, the conditionof the germplasm. Thus, any such methods using the S03-95341-A98-60618are part of this invention: selfing, backcrosses, recurrent selection,mass selection and the like.

Transformation methods are means for integrating new genetic codingsequences (transgenes) into the plant's genome by the incorporation ofthese sequences into a plant through man's assistance. Many dicotsincluding soybeans can easily be transformed with Agrobacterium. Themost common method of transformation after the use of agrobacterium isreferred to as gunning or microprojectile bombardment. This process hassmall gold-coated particles coated with DNA (including the transgene)shot into the transformable material. Techniques for gunning DNA intocells, tissue, explants, meristems, callus, embryos, and the like arewell known in the prior art. The DNA used for transformation of theseplants clearly may be circular, linear, and double or single stranded.Usually, the DNA is in the form of a plasmid. The plasmid usuallycontains regulatory and/or targeting sequences which assists theexpression of the gene in the plant. The methods of forming plasmids fortransformation are known in the art. Plasmid components can include suchitems as: leader sequences, transit polypeptides, promoters,terminators, genes, introns, marker genes, etc. The structures of thegene orientations can be sense, antisense, partial antisense, or partialsense: multiple gene copies can be used.

After the transformation of the plant material is complete, the nextstep is identifying the cells or material, which has been transformed.In some cases, a screenable marker is employed such as thebeta-glucuronidase gene of the uidA locus of E. coli. Then, thetransformed cells expressing the colored protein are selected for eitherregeneration or further use. In many cases, a selectable markeridentifies the transformed material. The putatively transformed materialis exposed to a toxic agent at varying concentrations. The cells nottransformed with the selectable marker, which provides resistance tothis toxic agent, die. Cells or tissues containing the resistantselectable marker generally proliferate. It has been noted that althoughselectable markers protect the cells from some of the toxic affects ofthe herbicide or antibiotic, the cells may still be slightly affected bythe toxic agent by having slower growth rates. If the transformedmaterial was cell lines then these lines are regenerated into plants.The cells' lines are treated to induce tissue differentiation. Methodsof regeneration of cellular are well known in the art. The plants fromthe transformation process or the plants resulting from a cross using atransformed line or the progeny of such plants are transgenic plantsthat carry the transgene.

Deposit Information

A deposit of the Advanta USA, Inc. Seed soybean cultivarS03-95341-A98-60618 disclosed above and recited in the appended claimswill be made with the American Type Culture Collection (ATCC), 10801University Blvd., Manassas, Va. 20110. The date of deposit was Nov. 24,2004. The deposit of 2,500 seeds maintained by Advanta USA, Inc. sinceprior to the filing date of this application. All restrictions on thedeposit upon issuance of the patent will be removed, and the deposit isintended to meet all of the requirements of 37 C.F.R. §§ 1.801-1.809.The ATCC accession number is PTA-6331. The viability of the deposit waspositive in tests ran on Dec. 1, 2004. The deposit will be maintained inthe depository for a period of 30 years, or 5 years after the lastrequest, or for the effective life of the patent, whichever is longer,and will be replaced as necessary during that period.

Accordingly, the present invention has been described with some degreeof particularity directed to the preferred embodiment of the presentinvention. It should be appreciated, though, that the present inventionis defined by the following claims construed in light of the prior artso that modifications or changes may be made to the preferred embodimentof the present invention without departing from the inventive conceptscontained herein.

1. A soybean seed designated S03-95341-A98-60618, representative seed of said soybean variety S03-95341-A98-60618 having been deposited under ATTC Accession No. PTA-6331.
 2. A soybean plant, or a part thereof, produced by growing the seed of claim
 1. 3. The soybean plant part of claim 2 wherein said part is pollen.
 4. The soybean plant part of claim 2 wherein said part is an ovule.
 5. A tissue culture of protoplasts or regenerable cells from the plant of claim
 2. 6. The tissue culture according to claim 5, the cells or protoplasts of the tissue culture are obtained from plant tissues selected from the group consisting of: leaf, pollen, cotyledon, hypocotyls, embryos, root, pod, flower, shoot and stalk.
 7. A soybean plant regenerated from the tissue culture of claim 5, having all the morphological and physiological characteristics of soybean variety S03-95341-A98-60618, representative seed of said soybean variety S03-95341-A98-60618 having been deposited under ATCC Accession No. PTA-6331.
 8. A method for producing a progeny soybean plant comprising crossing the plant of claim 2 with second soybean plant, harvesting the resultant soybean seed, and growing a progeny soybean plant. 